Standardization and Production Of Fungal Lipases By Solid State Fermentation Using The Selected Agro waste. Solid state fermentation is a process of producing the enzymes such as lipases using moist solid waste or residues from agro- industries and food industries. Solid state fermentation process was carried out by using three different substrates such as rice bran, coconut oil cake along with rice bran, groundnut oil cake along with rice bran along with the various carbon sources as substrate carried out various temperatures to best out the maximum production of lipase enzymes. Lipase production was found to be high in solid state fermentation than submerged fermentation. The optimum moisture content for lipase production was found at 55% and the suitable substrate was found to be groundnut oil cake and rice bran for both Aspergillus niger and Rhizopus. It was found that sucrose produced more lipase when it was assessed as a supplement in the medium. Among the nitrogen source used, yeast extract showed high yield of lipase. 1 % concentration of all carbon, nitrogen sources were proved to be effective in enhancing lipase production. Lipase enzyme activity was found be maximum on 9th day in both the microorganism. Aspergillus niger proved to be more effective than Rhizopus in enhancing the production of lipase.
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